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Plasmids Table.
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Image Search Results


Plasmids Table.

Journal: eLife

Article Title: The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response

doi: 10.7554/eLife.49577

Figure Lengend Snippet: Plasmids Table.

Article Snippet: This 3x-Flag dsDNA, with BglII-compatible sticky ends, was generated upon annealing of the two following single-stranded DNA fragments: 3x-Flag_UP: GATCTGGACTACAAGGACCATGACGGTGACTATAAGGATCACGACATCGACTACAAGGACGATGACGACAAG ; 3x-Flag_DOWN: GATCCTTGTCGTCATCGTCCTTGTAGTCGATGTCGTGATCCTTATAGTCACCGTCATGGTCCTTGTAGTCCA and it was mixed with a linearized and purified pPW3218, digested by BglI and dephosphorylated by Calf Intestinal Phosphatase (CIP, NEB) and incubated in presence of In-Fusion reagents (Takara) according to manufacturer’s instructions.

Techniques: Amplification, Sequencing, Clone Assay, Mutagenesis, Subcloning, Generated